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Image Search Results
Journal: Cancer biology & therapy
Article Title: Garlic constituent diallyl trisulfide induced apoptosis in MCF7 human breast cancer cells.
doi: 10.4161/cbt.8.22.9882
Figure Lengend Snippet: Figure 5. DATS treatment induced Bax expression in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM DATS for 24 h. Non-treated cells were used as control. (A) mRNA was extracted and reversed transcribed to cDNA for real-time PCR analysis of Bax mRNA levels (p < 0.05). (B) Bax in MCF-7 cells was immuno-stained using Bax (B-9) as the primary antibody and Texas Red—conjugated IgG as the secondary antibody, more details were described under the material and methods. The individual pictures for Bax were taken under a magnification of x200, using an immunofluorescence microscope (ECLIPSE E600; Nikon, Japan). Representative data from three indepen- dent experiments are shown.
Article Snippet: 3 μg of the total extracted RNA was subjected to RT-PCR and cDNA was synthesized using the Biorad
Techniques: Expressing, Control, Real-time Polymerase Chain Reaction, Staining, Immunofluorescence, Microscopy
Journal: Cancer biology & therapy
Article Title: Garlic constituent diallyl trisulfide induced apoptosis in MCF7 human breast cancer cells.
doi: 10.4161/cbt.8.22.9882
Figure Lengend Snippet: Figure 7. DATS modulates survival and apoptotic signals in MCF-7 human breast cancer cells. MCF-7 cells were treated with 100 μM of DATS for 24 h. Nested RT-PCR and real-time RT-PCR of total RNA isolated from MCF-7 cells were performed. Non-treated cells were used as control. The products of PCR were electrophoresed on 1.5% Agarose gels (A). mRNA was extracted and reversed transcribed to cDNA for RT-PCR analysis of mRNA levels (B). Each data point is an average of three independent experiments and expressed as M ± SD and the significance was calculated.
Article Snippet: 3 μg of the total extracted RNA was subjected to RT-PCR and cDNA was synthesized using the Biorad
Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Isolation, Control